Specification Brand : Botupharma USA BulletPoint1 : Contains Casein BulletPoint2 : Contains cholesterol to protect the sperm membrane and prevent premature capacitation. BulletPoint3 : Comparable to INRA 96 Color : Black/Gold ContainsLiquidContents : 1 Directions1 : How to Prepare: Carefully open the sachet, taking care not to touch the inside of the packet with your fingers, and empty the contents of the sachet into the bottle of sterile water. Replace lid on bottle, and shake well. Wait 30 minutes prior to using. This allows the casein to distribute properly for optimal results. Warm to 37°C before extending semen. Be sure extender remains at the same temperature as the semen to prevent cold shock of the semen. Directions2 : How to Use: The minimum dilution rate for preserving sperm motility during storage is 2 parts extender to 1 part semen, aiming for a final sperm concentration of 30 to 50 million total sperm per mL . Directions3 : IMPORTANT: In cases where centrifugation before the cooling process is necessary, re-suspend the sperm pellet at a final concentration of 100 mil- lion total sperm per mL. This ensures there is not too much cholesterol in the dose which could prevent the capacitation process of the sperm. Directions4 : Storage and stability: After mixing the contents of the sachet with sterile water, store it at 2 to 8°C (35.6 to 46.4°F) for up to 3 days, or aliqu Directions5 : WARNING: Keep out of reach of children and pets. ExternallyAssignedProductIdentifier : 7898626932026 FcShelfLife : 685 days IncludedComponents : One 200 mL diluent bottle containing sterile water and 1 sachet with 17 g. Ingredients : Casein, cholesterol, sugars, amino acids, antioxidants and excipients. IsExpirationDatedProduct : 1 ItemForm : Powder ItemName : BotuSemen Gold, 200 ml ItemPackageDimensions_Height : 9 inches ItemPackageDimensions_Length : 10 inches ItemPackageDimensions_Width : 7 inches ItemPackageQuantity : 1 ItemTypeKeyword : professional-medical-specimen-collection-containers LiquidVolume : 200 milliliters Manufacturer : Botupharma USA Inc ModelName : BotuSemen Gold, 200 mL NumberOfBoxes : 1 NumberOfItems : 1 ProductDescription : Botusemen Gold is a high fertility equine semen extender diluent that is suitable for all stallions, especially for those with suspected premature sperm capacitation. Can be used with fresh or cooled equine semen. Botusemen Gold is based on casein and cholesterol. Casein is a protein that interacts with calcium ions and prevents detrimental effects of seminal plasma semen by binding to HSP proteins, reducing lipid loss from the plasma membrane. Milk casein phosphoproteins protects sperm cells from the action of seminal plasma proteins (HSP-1, HSP-2) and Ca++ ions, prolonging spermatic longevity favoring semen transport. Reports show that seminal samples diluted in casein presents a higher rate of binding to the oocyte. The exclusive combination of casein and cholesterol in Botu Gold extender enhances the stability of the sperm membrane, preserving motility and viability during storage and cooling. Botusemen Gold increases the longevity and fertility rates of stallions' semen. ProductExpirationType : Expiration On Package ProductSiteLaunchDate : 2024-03-27T00:00:00.000-07:00 Size : 200 mL SpecificUsesForProduct1 : Stallion Semen Analysis SpecificUsesForProduct2 : Cooled and Shipped Semen SpecificUsesForProduct3 : Equine Semen Extender SpecificUsesForProduct4 : Centrifugation Extender StreetDate : 2024-10-04T07:00:00.000Z SupplierDeclaredDgHzRegulation : not_applicable TargetAudienceKeyword : Horses UnitCount : 6.73 UnspscCode : 10110000