Specification Brand : Thomas BulletPoint1 : Fine porosity and fast flow rate BulletPoint2 : 1.2 micrometer particle retention BulletPoint3 : 25 Seconds filtration speed BulletPoint4 : Primarily used for RIA procedures and harvesting lymphocytes BulletPoint5 : Binderless borosilicate glass microfiber ItemName : Thomas C1500 Borosilicate Glass Microfiber Filter, 1.2 Micron, Fast Flow, Grade C, 15cm Diameter (Pack of 100) ItemPackageDimensions_Height : 5.08 centimeters ItemPackageDimensions_Length : 16.764 centimeters ItemPackageDimensions_Width : 16.764 centimeters ItemPackageQuantity : 1 ItemTypeKeyword : science-lab-glass-fiber-filters Manufacturer : Thomas Scientific Material : Microfiber ModelNumber : C1500 NumberOfItems : 100 PartNumber : C1500 ProductDescription : Storage: 2-8°C UNSPSC Code: 12352200 RIDADR: NONH for all modes of transport Application: Collagenase from C. histolyticum is widely used for the disaggregation of many types of tissues ( e.g. , lung, heart, muscle, bone, adipose tissue, liver, kidney, cartilage, mammary gland, placentae, blood vessels, brain, tumors, umbilical cord) and for the preparation of single-cell suspensions for the establishment of primary cell culture systems. Collagenase A is recommended when yield, viability and functionality are important. Clostridium collagenase from Roche has been used to prepare cells from many types of tissue, such as hepatocytes, adipocytes, pancreatic islets, epithelial cells, muscle cells, endothelial cells, etc . However, suitability of each lot of the enzyme for disruption of a particular tissue should be determined empirically. General description: Collagenase is a protease which cleaves the triple-helical protein called collagen. Clostridium histolyticum produces six types of collagenases namely, +Ý, +Ý, +Ý, +Ý, +Ý and ++. These collagenases are active against connective tissue and show more reactivity towards gelatin than collagen. Collagenase obtained from Clostridium histolyticum has a very strong activity, as it digests collagen from both ends, at temperatures as low as 4-10 °C. Other Notes: For life science research only. Not for use in diagnostic procedures. Preparation Note: Activator: Ca 2+ Inhibitors: Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercapto-ethanol Collagenase is not inhibited by serum. Clostripain inhibitors: TLCK Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean), serum Working concentration: 0.5 to 2.5 mg/ml Storage conditions (working solutio ProductSiteLaunchDate : 2012-01-15T00:00:01-08:00 Size : 1.2 micrometer Retention, Fast Flow, Grade C, 0.28 mm Thickness, 15cm Diameter (Pack of 100), StreetDate : 2017-05-15T00:00:01Z SupplierDeclaredDgHzRegulation : not_applicable UnitCount : 100 UnspscCode : 41000000